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Sulfhydryl groups have the ability to react with the alkylating reagent N-ethylmaleimide (NEM) in chemical modification experiments. When NEM is added to a purified solution of creatine kinase, Cys 278 is alkylated, but no other Cys residues in the protein are modified. What can you infer about the Cys 278 residue based on this observation?
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