生态学调查数据常面临零膨胀、厚尾分布和小样本等挑战，直接进行两两t检验易导致假阳性率大幅上升。理解组间差异需要从α多样性、β多样性和差异物种三个维度切入：α多样性反映各组内部丰富度，β多样性借助Bray-Curtis距离和置换检验评估群落组成差异，PERMANOVA作为多组别比较的事实标准 ...
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